How to Spend Your Weekend – With Nematodes

An amusing add directed to American livestock farmers that has appeared in several agriculture and livestock magazines –

Source - DECTOMAX

Advice from Steer Wrestler Matt Reeves, a DECTOMAX user.  Source – DECTOMAX

m-and-f-nematodes-non-parasitic-and-a-close-up-of-a-nematode-mouth-right  Source - www.marlin.ac.uk

Male and female nematodes – and a close-up of a nematode mouth, right.  Source – http://www.marlin.ac.uk

Nematodes are one of the biggest problems not only in the States, but globally.  The increasing over-use and inappropriate use of anthelmintics , however, is  leading to serious resistance and thus to uselessness or greatly diminished efficacy of the products.

See this link for a short video of the Anthelmintic effect of papain on Heligmosomoides bakeri (the parasite shown in the picture, below). This is a Wikipedia page, you will need to scroll down and on the right side you will see the black and picture shown below, which you will then open to view the short video.

http://upload.wikimedia.org/wikipedia/commons/thumb/f/f1/Anthelmintic_effect_of_papain_on_Heligmosomoides_bakeri.ogv/384px–Anthelmintic_effect_of_papain_on_Heligmosomoides_bakeri.ogv.jpg

A still from the video.  Source - anthelmintic_effect_of_papain_on_heligmosomoides_bakeri-ogv

A still from the video. See below for explanation.  Source – anthelmintic_effect_of_papain_on_heligmosomoides_bakeri-ogv

A single adult female living specimen of Heligmosomoides bakeri was mounted on a microscope slide in Hanks’s saline and sandwiched beneath a glass coverslip supported on petroleum jelly. The worm was imaged using a Zeiss Axiovert 135TV inverted microscope and photographed using a Scion CFW 1310 M digital camera. A solution of 25 μM papain was introduced below the coverslip and images were captured on a PC using Streampix III time-lapse software at a frame rate of approximately 1 image every 3 seconds for 30 minutes. The video file was edited and exported as an mpeg running at 10 times the original speed. The file is titled “H. bakeri female papain.mpg” and initially shows the worm freely moving in the papain solution. After the animal forms a helical coil, a lesion appears on the left of the worm. This is followed by rupture of the worm and loss of the viscera through the rupture leading to the death of the parasite

Source – Behnke, J. M.; Buttle, D. J.; Stepek, G.; Lowe, A.; Duce, I. R. (2008). “Developing novel anthelmintics from plant cysteine proteinases”. Parasites & Vectors 1: 29.doi:10.1186/1756-3305-1-29.

Celegans Goldstein, Laboratory at University of North Carolina. Source - Wikipedia

A healthy helminth, or roundworm, Celegans Goldstein, Laboratory at University of North Carolina. Source – Wikipedia

source - www.wormbook.org

Wild Types of nematode.  Source – http://www.wormbook.org

Here are a couple of online resources that are pretty good –

The Worm Breeder’s Gazette

Some recent articles –
Nematode news in brief
Decrepitus W. PickerPre-approval of strains donated to the CGC
Aric Daul and Ann RougvieEndogenous locus modification by genome engineering: proposed nomenclature
Tim Schedl and Jonathan HodgkinAn online OrthoList tool to query worm-human gene homology
Daniel Shaye, Woojin Kim, James Chen and Iva GreenwaldProtein extraction from C. elegans using sea sand
Shamol Saha, Julien Dusonchet and Benjamin WolozinUsing magnetism to move C. elegans
Peter Wawrzusin, Yicong Wu, Peter Winter and Hari ShroffLive worm respiration in 24-well format
Beverley M. Dancy, Ernst-Bernhard Kayser, Margaret M. Sedensky and Philip G. Morgan… etc.

The WormBook

A comprehensive, open-access collection of original, peer-reviewed chapters covering topics related to the biology of Caenorhabditis elegans and other nematodes.Wormbook also contains: WormMethods, a collection of protocols for nematode researchers; WormHistory, personal perspectives on C. elegans research; and the Worm Breeder’s Gazette, an informal, non-refereed, biannual newsletter for the interchange of ideas and information related to C. elegans and other nematodes.

The Woprm Breeders Gazette / http://www.wormbook.org/wbg/

The Worm Breeders Gazette / http://www.wormbook.org/wbg/

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About dianabuja

With a group of BaTwa (pygmy) women potters, with whom we've worked to enhance production and sales of their wonderful pots - fantastic for cooking and serving. To see the 2 blogs on this work enter 'batwa pots' into the search engine located just above this picture. Blog entries throughout this site are about Africa, as well as about the Middle East and life in general - reflecting over 35 years of work and research in Africa and the Middle East – Come and join me!
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